TY  - JOUR
T1  - Characterization of Interferon-α1 of Yak
AU - Jing, ZhiZhong AU - Cai, XuePeng AU - He, XiaoBing AU - Fang, YongXiang AU - Zeng, Shuang AU - Zhen, YaDong AU - Jia, HuaiJie AU - Chen, GuoHua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 21
SP  - 3978
EP  - 3983
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3978.3983
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3978.3983
KW  - cloning
KW  -interferon-a1
KW  -Yak (Bos grunniens)
KW  -antiviral activity assay
KW  -Pichia pastoris
AB  - The yak (<I>Bos grunniens</I>) is an important grazing livestock in Western of China. In this study, the full length of <I>yIFN-a1</I> gene was cloned then the recombinant yIFN-a1 was expressed in yeast expression system. <I>In vitro</I> bioactivity of IFN-&#945;1 was determined by inhabition infection BHK cell against Vesicular Stomatitis Virus (VSV). The results showed that cDNA contains an Open Reading Frame (ORF) of 570 base pairs encoding a protein of 189 amino acid (aa) precursor with a signal peptide of 23 aa. The homology of the yIFN-a1 was up to 65.3~94.4% with other species at the amino acid levels. The recombinant yIFN-a1 was recognized in Western blot by mAb against human IFN-&#945;1. The activity unit of the purified recombinant yIFN- a1 protein was 1.16&times;10<SUP>5</SUP> IU mg<SUP>-1</SUP> by against Vesicular Stomatitis Virus (VSV) antiviral assay <I>in vitro</I>. This made a basis for the development of anti-virus medicine of yak in the future.
ER  -