TY  - JOUR
T1  - Characterization of &#946;-Actin Promoter of <I>Leuciscus merzbacheri</I>
AU - Ma, Shiwei AU - Hu, Wenge AU - Chen, Chuangfu AU - Wang, Yuanzhi AU - Ren, Yan AU - Cao, Xudong AU - Sheng, Jinliang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 20
SP  - 3849
EP  - 3854
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3849.3854
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3849.3854
KW  - Leuciscus merzbacheri
KW  -promoter analysis
KW  -B-actin gene
KW  -green fluorescent protein
KW  -China
AB  - <I>Leuciscus merzbacheri</I> is a unique vulnerable indigenous 
  fish which is only distributed in the Junggar Basin, Xinjiang. In this study, 
  researchers cloned two <I>L. merzbacheri</I> &#946;-actin promoter fragments 
  of different length: SZ11 and SZ21 and analyzed their structural features. The 
  mammalian expression vector pEGFP-N1 was used to construct eukaryotic expression 
  vectors &#946;1 pEGFP-N1-AFP III and &#946;2 pEGFP-N1-AFP III in which fish 
  type III antifreeze protein gene was used as the structural gene. The results 
  showed that the cloned two fragments of &#946;-actin promoters had the ability 
  to drive the expression of the green fluorescent protein gene in BHK-21 cells. 
  These data suggest that the vectors researchers constructed based on &#946;-actin 
  promoter could be exploited as all fish recombinant eukaryotic expression vector.
ER  -