TY  - JOUR
T1  - Rapid Detection of <I>Edwardsiella ictaluri</I> from Channel Catfish Tissue and Water Samples by PCR Amplification
AU - Sanchez-Martinez, Jesus Genaro AU - Perez-Castaneda, Roberto AU - Vazquez Sauceda, Ma. De la Luz AU - Castro, Jaime Rabago AU - Aguirre-Guzman, Gabriel 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 20
SP  - 3823
EP  - 3826
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3823.3826
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3823.3826
KW  - PCR
KW  -detection
KW  -E. ictaluri
KW  -catfish
KW  -Ell6s
AB  - Polymerase Chain Reaction (PCR) primers were designed to detect 
  <I>Edwardsiella ictaluri</I> in tissue and water samples. Three pairs of primers 
  were designed and tested against the <I>E. ictaluri</I> type strain (ATCC No. 
  33202). The primers for pEI1 and EIl6s were very sensitive for the detection 
  of <I>E. ictaluri</I> in water and tissue samples of fish experimentally infected 
  with different bacterial dilutions. The PCR conditions described were optimized 
  to obtain results in 5 h. This PCR has the potential of detecting low levels 
  of <I>E. ictaluri</I> in tissue and water samples allowing for the timely detection 
  of enteric septcaemia of catfish in farms.
ER  -