TY  - JOUR
T1  - Prokaryotic Expression of <I>p1</I> Gene of <I>Yersinia ruckeri</I> Isolated 
  from Channel Catfish (<I>Ictalunes punctatus</I>) and Optimization of Expression Conditions
AU - Wang, Kai-Yu AU - Lian, Hai AU - Chen, De-Fang AU - Wang, Jun AU - Huang, Ling-Yuan AU - Li, Cheng-Wei 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 20
SP  - 3800
EP  - 3805
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3800.3805
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3800.3805
KW  - Yersinia ruckeri
KW  -p1 gene
KW  -prokaryotic expression
KW  -optimization
KW  -China
AB  - The <I>p1</I> gene of <I>Yersinia ruckeri</I> (<I>yrp1</I>) 
  which was isolated from channel catfish was amplified by PCR with specific primers 
  and inserted into pMD19-T vector. The positive recombinant plasmid was selected 
  and sequenced. Then, the <I>yrp1</I> gene was subcloned into pET-32a (+) vector 
  and transformed into BL21 (DE3) followed by induction with IPTG and detection 
  with SDS-PAGE. Optimization of the induction conditions were conducted. The 
  results showed that the recombinant protein with a molecular mass of about 72 
  kDa was mostly packaged into inclusion bodies. The optimization of induction 
  process conditions led us to perform the fusion protein induction at 37&deg;C 
  for 4 h with 0.8 mM IPTG.
ER  -