TY  - JOUR
T1  - Cloning and Sequence Analysis of the Complete Genome of Porcine Torque Teno Sus Virus Type 1
AU - Wanzhu, Guo AU - YaLin, He AU - Ling, Zhu AU - Xiaoke, Feng AU - Ling, Zhao AU - Mengjin, Fu AU - Miao, Mei AU - Yunfei, Wu AU - Wen, Xu Zhi- 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 20
SP  - 3755
EP  - 3760
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3755.3760
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3755.3760
KW  - Porcine torque teno Sus virus type 1
KW  -cloning
KW  -sequence analysis
KW  -virus
KW  -genetic variation
AB  - According to the published genomic sequence of TTSuV1 (GenBank 
  Accession No. AB076001.1), the pairs of specific PCR/nested-PCR primers were 
  designed to amplify the full length of TTSuV1 complete genome from blood serum 
  samples in Sichuan province. The amplified fragments were cloned, sequenced 
  and named as TTSuV1-SC1 and TTSuV1-SC2, respectively. The sequencing results 
  showed that the complete genome of them were 2852 bp and 2917 bp in length. 
  And then, the correct target sequences gained were deposited in GenBank and 
  the GenBank accession number JF694116 and JF694117 were generated. Homology 
  analysis of TTSuV1-SC1, TTSuV1-SC2 and reference strain sequences retrieved 
  in GenBank indicated that the complete genome of TTSuV1-SC1 and TTSuV1-SC2 from 
  Sichuan isolated strains shared 81-98 and 82-99% homology, respectively with 
  those reference sequences. The phylogenetic tree analysis indicated that the 
  closest genetic relationship of TTSuV1-SC1 and TTSuV1-SC2 isolated strains were 
  TTV1Bj10 strain HM633251.1 and TTVSH0822/2008 strain (GU450331.1). The study 
  would lay a biological foundation to understand the epidemiology, genetic variation 
  of the virus.
ER  -