TY  - JOUR
T1  - Allelic Polymorphism Identified and Analysis on the Fifth Exon of Chinese Indigenous Donkey <I>GH</I> Gene by PCR-SSCP
AU - Zhu, Wenjin AU - Su, Yongmei AU - Liu, Yanfang AU - Guan, Xuemin AU - Ni, Jing AU - Wu, Jianhua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 20
SP  - 3713
EP  - 3716
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3713.3716
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3713.3716
KW  - Donkey
KW  -GH gene
KW  -exon3
KW  -polymorphism
KW  -PCR-SSCP
AB  - Growth of animal is largely regulated by Growth Hormone (GH). 
  In this study the objective was to investigate variations in the fifth exon 
  of <I>GH</I> gene and their polymorphism in 5 Chinese indigenous donkeys by 
  PCR-SSCP and DNA Sequencing Methods. The results showed that there was a transversion 
  at nucleotide position 1802 of <I>GH</I> gene in the 5th exon named as GH-exon5-G1802C 
  which led to a conservative Lysine to Asparagine substitution at amino acid 
  position 205. In detail, two different alleles, A and B were identified and 
  three genotypes were observed, AA, AB and BB with the frequency distribution 
  of allele B from 0.5962-0.7344 in analyzed populations. The genetic diversity 
  analysis revealed that all PIC values were between 0.3140 and 0.3656, implying 
  that this locus within <I>GH</I> gene possessed moderate genetic diversity in 
  5 Chinese indigenous donkeys. The &#967;<SUP>2</SUP>-test showed that GZ donkey 
  was significant deviation, DZ donkey was no significant deviation from Hardy-Weinberg 
  equilibrium, GL, JN and XJ donkey was not in agreement Hardy-Weinberg equilibrium. 
  The results confirmed that there were polymorphisms in the exon 5 of <I>GH</I> 
  gene.
ER  -