TY - JOUR T1 - Effects of Dimethyl Sulfoxide, Ethylene Glycol, Propylene Glycol and Glycerol on Cryopreservation of Wild Tree Shrew (Tupaia belangeri Chinese) Cauda Epididymal Sperm AU - Yang, Shi-Hua AU - Ping, Shu-Huang AU - Yue, Feng AU - Wang, Cai-Yun AU - Luo, Ying AU - Si, Wei JO - Journal of Animal and Veterinary Advances VL - 11 IS - 19 SP - 3568 EP - 3574 PY - 2012 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2012.3568.3574 UR - https://makhillpublications.co/view-article.php?doi=javaa.2012.3568.3574 KW - Tree shrew KW -sperm KW -cryopreservation KW -cryoprotectant KW -DMSO KW -China AB - The objective was to examine the effect of Permeable Cryoprotectant Agents (CPAs) on sperm cryopreservation of tree shrew. Epididymal sperm were surgically harvested from captured wild male tree shrews and cryopreserved with Tes-Tris-Egg yolk based cryodiluent (TTE) containing either of the four CPAs, Dimethyl Sulfoxide (DMSO), Ethylene Glycol (EG), Propylene Glycol (PG) and Glycerol (Gly) at concentrations of 1, 3, 6 and 10%, respectively. Sperm motility, acrosome integrity and fertility were assessed. In Experiment 1, sperm equilibrated at 4°C in TTE containing 1, 3 and 6% DMSO, respectively showed similar motility to that in TTE without CPA (p>0.05). Following the increase of concentration of CPAs and equilibration time (30-90 min), the other CPAs reduced sperm motility (p<0.05). In Experiment 2, sperm frozen in TTE containing 3% DMSO showed the highest post-thaw motility (p<0.05) and recovery rate of motility (p<0.05) among groups. In Experiment 3, there were no differences in the fertilization rate of oocytes and the proportion of tree shrews yielding fertilized oocytes inseminated with fresh and thawed sperm frozen in TTE containing 3% DMSO (p>0.05). In conclusion, among the permeable CPAs tested, DMSO provided the best cryoprotective ability for captured wild tree shrew epididymal sperm. ER -