TY  - JOUR
T1  - Comparison of Intradermal Tests, Total Serum IgE Concentrations and Allergen-Specific 
  IgE Using an Arrayed Protein Chip in Atopic Dogs
AU - Huang, Hui-Pi AU - Lo, Pei-Ying AU - Chen, Kang-Hsin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 18
SP  - 3298
EP  - 3303
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3298.3303
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3298.3303
KW  - Atopic dermatitis
KW  -arrayed protein chip
KW  -dog
KW  -IgE
KW  -serum
KW  -screening
AB  - Canine atopic dermatitis is a common pruritic skin disease which is most commonly associated with Immunoglobulin (Ig) E immune response to environmental allergens. The aims of this study were to report comparison between total serum IgE concentrations in atopic and non-atopic dogs. Comparison between results of intradermal tests and serum allergen-specific IgE arrayed protein chips was also investigated. Total serum IgE concentrations between 37 atopic dogs (92.5&plusmn;102.3 &#956;g mL<SUP>-1</SUP>) and 34 non-atopic dogs (16.2&plusmn;30.2 &#956;g mL<SUP>-1</SUP>) were significantly different (p&lt;0.001). A positive correlation between total serum IgE concentrations and corresponding modified canine atopic dermatitis extent and severity index scores was found (r = 0.56, p&lt;0.001) in atopic dogs. The cut-off value was 16.8 &#956;g mL<SUP>-1</SUP> with the sensitivity of 91.9% and the specificity of 85.3%. The overall sensitivity of specific IgE serum tests was 24%, specificity was 95% and efficacy was 81%. Total serum IgE concentrations could be a screening test and determine severity of atopic dermatitis in dogs with discrete clinical evaluation. However, arrayed protein chip method might not be suitable for screening functional allergen-specific IgE.
ER  -