TY  - JOUR
T1  - Antibacterial Activity and Mechanism of <I>Pogostemon cablin</I> Against Bacteria from Milk of Dairy Cows Suffering with Mastitis
AU - Peng, Cheng AU - Dai, Min AU - Wan, Feng AU - Peng, Fu 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 18
SP  - 3289
EP  - 3297
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3289.3297
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3289.3297
KW  - Pogostemon cablin
KW  -essential oil
KW  -bovine mastitis
KW  -in vitro and in vivo antibacterial activity
KW  -antibacterial mechanism
KW  -China
AB  - Minimum Inhibitory Concentrations (MICs) of 3 extracts and oil from <I>Pogostemon cablin </I>against isolates from milk produced by dairy cow with mastitis were determined by Agar Dilution Method. Water extract had low activity; ethanol extract and ethanol-water extract had moderate effects having MICs ranging from 25.0-100.0, 12.5-100.0 mg mL<SUP>-1</SUP>, respectively. Oil had strong activity against Gram-positive and Gram-negative isolates with MICs ranging from 0.2-8.2 mg mL<SUP>-1</SUP>. Oil also, showed strong efficacy in mouse peritonitis model infected with <I>S. aureus</I> or <I>E. coli</I>. Their actions were dose-dependent. Obvious morphological changes were observed by TEM in oil-treated <I>E. coli</I> or <I>S. aureus</I> such as a remarkable electron-light region small electron-dense granules disturbed cytoplasmic membrane and abnormal cell division. In conclusion, antibacterial substance of <I>P. cablin</I> was oil which had broad-spectrum antibacterial activities so to make it a potential candidate as antibacterial agent to treat bovine mastitis.
ER  -