TY  - JOUR
T1  - Detection of Brucella among Voluntary Blood Donors in Turkey by Using a New Real Time PCR Method
AU - Yavuz, M. Tevfik AU - Kaya, Demet AU - Behcet, Mustafa AU - Aksit, Hasan 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 17
SP  - 3247
EP  - 3250
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3247.3250
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3247.3250
KW  - Brucella
KW  -blood transmission
KW  -real time PCR
KW  -donor
KW  -agglutination
AB  - Brucellosis is a worldwide zoonosis also the incidence of 
  which has decreased in developed countries it is still effective in Asia, Africa, 
  Central America and the Mediterranean basin (Turkey inside) that have insufficient 
  public health and domestic animal health programs. There are many reports about 
  Brucella endemics in Turkey. Although, human to human transmission is not frequently, 
  it has been reported in association with blood transfusion, bone marrow transplantation 
  and transplacental transmission in worldwide and Turkey. Human brucellosis is 
  diagnosed based on clinical findings and laboratory studies that include bacteriological 
  and serological tests. The conventional serological tests are insufficiently 
  sensitive and specific to be used individually and often leading to difficulty 
  in interpreting the results. In this study, researchers aimed the detection 
  of possible Brucella carriers without manifest clinical finding among voluntary 
  blood donors by using new real time PCR Method. Total 632 voluntary donor blood 
  samples for four month were investigated for Brucella. The samples were subjected 
  to both Wright and ELISA tests for Brucella. In the second step, leucocytes 
  were separated from all samples and performed real time PCR based on BioRad 
  I-Cycler. Although, the region is not high risk epidemiologic area for Brucellosis 
  in Turkey 4 out of total 632 samples were detected by Wright agglutination and 
  Brucella IgG, IgM ELISA while 2 positive samples were found by I-Cycler based 
  real time PCR detection. All of positive samples were collected from rural area 
  donors. Even it is not in high prevalence, this results and earlier studies 
  were shown brucellosis could be a risk for blood transfusion. Researchers advice 
  especially donors from coming rural area must be performed a questionnaire for 
  brucellosis findings and Brucella screening tests must be performed for blood 
  donors in endemic areas.
ER  -