TY  - JOUR
T1  - Effects of Skim Milk and Tris Extender on Frozen-Thawed Canine Sperm Morphology
AU - Ozdas, Ozen Banu AU - Baran, Alper AU - Sandal, Asiye Izem AU - Ak, Kemal 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 17
SP  - 3242
EP  - 3246
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3242.3246
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3242.3246
KW  - Canine
KW  -semen
KW  -freezing
KW  -extender
KW  -post-thaw
KW  -abnormal spermatozoa
AB  - This study was aimed to compare the effects of freezing dog 
  semen in straws in 0.1% fat bearing Skim Milk-Glucose (SMG) extender with the 
  routinely used TRIS-Fructose-Citric acid (TFC) extender regarding the post-thaw 
  motility, acrosome and total morphologic defects rate. Four German shepherd 
  male dogs of 2-3 years under the same managemental conditions on a private kennel 
  were used. Six ejaculates per each dog (total 24 ejaculates) were collected 
  digital manipulation. Two extenders used were 20% egg yolk containing TFC and 
  10% egg yolk containing SMG extenders. Semen was divided into two split parts 
  and one was extended with TFC and the other with SMG extenders at 26°C 
  at a rate 1:1 and cooled to 5°C in an hour. Cooled semen samples were further 
  extended with 10% (v/v) glycerol containing extender (for an hour) at equal 
  rates (final glycerol rate 5%). Following glycerolisation semen samples were 
  filled into 0.5 mL straws and equilibrated for an hour at 5°C. Straws were 
  frozen at –110°C liquid nitrogen vapour for 7 min. Labeled straws were 
  stored in separate canisters in liquid nitrogen at –196°C. Six straws 
  for TFC extender and six straws for SMG extender were stored to be examined. 
  Straws were thawed at 45°C for 60 sec. Post-thaw motility rate of TFC and 
  SMG extended semen samples were, respectively 48.54±8.27 and 51.97±7.51%. 
  Acrosome and total morphologic defect rates were 41.04±9.44 and 51.17±9.05% 
  for TFC; 38.04±13.60 and 46.67±12.68% for SMG extender, respectively. 
  It has been founded that when the post-thaw sperm traits of straws frozen dog 
  semen was evaluated, SMG extender have been successful at least as much as TFC 
  extender.
ER  -