TY  - JOUR
T1  - Immunogenicity of Recombinant Attenuated Salmonella Choleraesuis C500 Strain Co-Expressing M and N Protein of Porcine Epidemic Diarrhea Virus (PEDV)
AU - Yan, Qi-Gui AU - Ren, Yu-Peng AU - Wang, Xiao-Yu AU - Chen, Di-Shi AU - Cheng, Yu AU - Wan, Li AU - Ma, Lei AU - Zhang, Qi AU - Guo, Lin AU - Xie, Zhi-Yong AU - Guo, Wan-Zhu 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 17
SP  - 3234
EP  - 3241
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3234.3241
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3234.3241
KW  - PEDV
KW  -membrane protein
KW  -nucleocapsid protein
KW  -attenuated salmonella
KW  -immune response
KW  -China
AB  - This study examined the recombinant Attenuated Salmonella 
  Choleraesuis C500 containing <I>M</I> and <I>N</I> gene of PEDV whether can 
  induce a systemic immune responses in mice. After identified by enzyme analysis 
  and nucleotide sequencing test, the constructed recombinant plasmid, designated 
  as pIRES-M-N was transfected into Vero cells. The transient expression protein 
  was detected by indirect Immunofluorescence Assay (IFA) and Western-blotting. 
  Result showed that pIRES-M-N can be expressed correctly in Vero cells. Then, 
  the recombinant plasmid electroporated into <I>S. choleraesuis</I> C500 and 
  the recombinant bacteria was proved to be stabile <I>in vitro</I>. The immunogenicities 
  of this recombinant attenuated salmonella constructs were firstly investigated 
  in a mouse mode. Specific anti-PEDV IgG antibodies and IFN-&#947;, IL-4 of cytokine 
  were detected. The result showed that the recombinant Attenuated Salmonella 
  could induce higher persistence specific hummune responses in mouse by contrast 
  with naked DNA group after 42 days (p&lt;0.05). It also, stimulated a balanced 
  Th1/Th2 immune response. That provided the clinical basic data and theoretical 
  basis for success of the attenuated salmonella vaccine development.
ER  -