TY  - JOUR
T1  - Cloning and Sequence Analysis of Rabbit Interleukin-10 (<I>IL-10</I>) Gene Promoter
AU - Xin-Sheng, Wu AU - Meng-Jie, He AU - Lei, Zhang AU - Kai, Feng AU - Xiao-Ming, Wang AU - Yu-Lai, Pan AU - Xiao-Ying, Wan AU - Liu-Liu, Mao 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 3
SP  - 323
EP  - 326
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.323.326
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.323.326
KW  - bioinformatics
KW  -transcription factor
KW  -promoter
KW  -IL-10
KW  -gene
KW  -China
AB  - To clone the IL-10 promoter of rabbit and analyse its sequence, this study amplified the 5 flanking region of rabbit IL-10, compared the genomes and used bioinformatics software to make a forecast. Then, the researchers comfirmed partial promoter sequences and analysed some transcription factors of the <I>IL-10</I> gene promoter. The study showed that rabbit and human <I>IL-10</I> gene promoter had some similarities which all cotained GAGA, CAAT and TATA. The transcriptional start site started from -80 bp of translation initiation codon (ATG) upstream region. The promoter also had some transcription factors such as AP-1, Myogenin, c-Ets-1, C/EBP alpha and so on. Results of this study predicted partial sequences, transcriptional start site and some transcriptional regulating factors binding sites of <I>IL-10</I> gene promoter which would lay the foundation of lucubrating the expression and regulation of rabbit <I>IL-10</I> gene.
ER  -