TY  - JOUR
T1  - Immunoregulation of<i> Lycium barbarum</i> Polysaccharide in Vaccinated 
  Chickens
AU - Qin, Ruiling AU - Chu, Yaocheng AU - Lian, Junfeng AU - Xu, Zhanyun AU - Li, Chunhong AU - Wang, Li AU - Li, Guohui 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 17
SP  - 3105
EP  - 3110
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3105.3110
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3105.3110
KW  - Lycium barbarum polysaccharide
KW  -vaccinated chickens
KW  -antibody
KW  -IL-2
KW  -T lymphocyte
KW  -ratio of CD4+ and CD8+T lymphocyte
KW  -immune organs
KW  -China
AB  - The objective of the present study was to evaluate the effects 
  of <I>Lycium barbarum</I> Polysaccharide (LBP) on immune responses in vaccinated 
  chickens. A total of 600 Hy-Line Brown chickens aged 15 days old were randomly 
  divided into four groups with three replicates per group and fifty chickens 
  per replicate and all the chickens were injected with Newcastle Disease (ND) 
  vaccine. Three experimental groups of chickens were injected with LBP 20, 10 
  and 5 mg kg<SUP>-1</SUP> (LBP<SUB>H</SUB>, LBP<SUB>M</SUB> and LBP<SUB>L</SUB>) 
  and the control group were injected with equal dose normal saline (0.09% NaCl), 
  respectively per day for 7 days. On the 7, 14, 21, 28, 35 and 42 days after 
  vaccination, ten chickens were sampled randomly from each group and the serum 
  was seperated for the determination of NDV-HI antibody titers by Micro Method. 
  On day 10, 20, 30, 40 and 50 after vaccination, blood samples from 5 chickens 
  per group were collected to separate lymphocyte and determine the peripheral 
  blood T lymphocyte proliferation with Methyl Thiazolyl Tetrazolium (MTT) Method. 
  The content of CD4+ and CD8+ T cells were tested by using flow cytometry with 
  Double Color Staining Method. The IL-2 levels were determined by ELISA Method 
  and the whole body, bursa of fabricius and thymus were weighted for calculation 
  of immune organ index. The results showed that LBP (10 and 20 mg kg<SUP>-1</SUP>) 
  could significantly raised the ratio of CD4+ and CD8+T lymphocyte (p&lt;0.01) 
  and the production of IL-2. It also significantly enhanced the ND antibody titers, 
  promoted the proliferation of peripheral blood T lymphocyte and increased immune 
  organ indexes (p&lt;0.01). These results indicated that LBP had significant 
  immunoregulation functions of ND vaccine in chickens.
ER  -