TY  - JOUR
T1  - Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 Strain
AU - Wang, Hong-Ning AU - Fan, Wen-Qiao AU - Zhang, Yi AU - Zhang, An-Yun 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 17
SP  - 3064
EP  - 3067
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3064.3067
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3064.3067
KW  - Avian infectious bronchitis virus
KW  -housekeeping genes
KW  -quantitative real-time reverse transcription-polymerase chain reaction
KW  -suitability
KW  -China
AB  - Avian Infectious Bronchitis (IB) is an acute and highly contagious 
  respiratory disease in chickens. To detect the virus in chickens, housekeeping 
  genes were detected by quantitative real-time PCR for the normalization. The 
  aim of study was to identify the most stable housekeeping gene (s) in IBV infection. 
  Five housekeeping genes including <I>18S rRNA</I>, <I>&#946;-actin</I>, Glyceraldehyde-3-Phosphate 
  Dehydrogenase (<I>GAPDH</I>), Glucose-6-Phosphate Dehydrogenase (<I>G6PDH</I>) 
  and Ubiquitin (<I>UB</I>) were investigated in nine tissues: trachea, thymus, 
  liver, spleen, lungs, kidney, pancreas, proventriculus and bursa of Fabricius. 
  The geNorm and Normfinder Software were used to calculate the stability of the 
  candidate genes.
ER  -