TY  - JOUR
T1  - Preparing Rat Spermatogonial Transplantation Recipient by Treating the Pregnant Female Rats with Busulfan
AU - Wu, Yingji AU - Yu, Boyang AU - Luo, Fenhua AU - Liu, Taodi AU - Liu, Daiyan AU - Liu, Linhong AU - Zhang, Yan 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 17
SP  - 3036
EP  - 3044
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3036.3044
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3036.3044
KW  - Busulfan
KW  -germ cells
KW  -infertility
KW  -rat
KW  -spermatogonial transplantation
AB  - Successful spermatogonial stem cells transplantation is largely dependent on the preparation of the recipient. In the present study, to acquire more efficient rat recipients, the response of pregnant female rats to treatment with 7.5-12.5 mg busulfan per kg of body weight was determined in terms of the birth rate, survival rate, body weight, testicular mass, histology, expression of certain germ cell genes as measured by real time PCR and fertility rate of male offspring. The analysis suggested that a dosage of 10 mg busulfan per kg administered to pregnant female rats at 13.5 day post pregnancy effectively prepares male pups to be recipients because it leads to the maximal deletion of endogenous spermatogonial stem cells with minimal effects on the recipients’ health.
ER  -