TY  - JOUR
T1  - The Nucleocapsid Protein of Avian Infectious Bronchitis Virus Interacts with Chicken Ubiquitin-Conjugating Enzyme 9 (cUBC9)
AU - Wang, Hongning AU - Zhao, Sujun AU - Fan, Wenqiao AU - Yang, Xin AU - Zhang, Anyun AU - Zeng, Bo AU - Zhang, Yi 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 16
SP  - 2957
EP  - 2963
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2957.2963
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2957.2963
KW  - IBV
KW  -nucleocapsid protein
KW  -cUBC9
KW  -interaction
KW  -yeast two-hybrid
KW  -coimmunoprecipitation
AB  - Avian Infectious Bronchitis Virus (IBV) is an avian coronavirus. The Nucleocapsid (N) protein of IBV (IBV-N) is an important structural and functional protein and plays an essential role throughout the virus life cycle. Some cellular proteins may be involved in these complicated processes, little is known about virus host interactions. In this study, host cell proteins that interact with N protein were sought by screening a chicken lung cDNA library using a yeast two-hybrid system assay. IBV-N was shown to interact specifically with chicken Ubiquitin-Conjugating enzyme 9 (cUBC9) which belongs to the ubiquitin-conjugating enzyme family and is an important enzyme in the sumoylation system. Furthermore, the interaction between IBV-N and cUBC9 was confirmed by coimmunoprecipitation. The results of mapping the cUBC9 binding region of IBV-N indicated that N151-230 fragment of IBV-N is responsible for the majority of the binding to cUBC9. This virus-host protein interaction may provide insights into the function of IBV-N and facilitate rational targeting of the viral nucleocapsid protein for the development of structure-based vaccines and antiviral compounds.
ER  -