TY  - JOUR
T1  - Comparison of Adipose Tissue Derived Stem Cells from Different Subcutaneous Adipose Tissue Depots of Canine
AU - Yang, Jing AU - Cai, Xiangang AU - Song, Tao AU - Chen, Yongjun AU - Chen, Hui AU - Wu, Pan AU - Huang, Congxin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 16
SP  - 2845
EP  - 2851
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2845.2851
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2845.2851
KW  - Subcutaneous adipose tissue
KW  -mesenchymal stem cells
KW  -canine adipose tissue derived stem cells
KW  -tissue engineering
KW  -transduction
AB  - Adipose-derived Stem Cells (ASCs) have great potential in the field of tissue engineering. There is evidence that human ASCs from different depots of adipose tissue may produce different characteristics. Canine is a large and important animal model for human diseases which has led to interest in the isolation and characterization of canine ASCs. Canine Adipose tissue derived mesenchymal Stem Cells (cASCs) also have been shown to possess the proliferation and multi-differentiate capacity. The main goal of this research was to compare the proliferation capacity, phenotypes, apoptosis susceptibility, differentiation capacity and gene transfection efficiency of cASCs obtained from different depots (superficial abdominal, hip, thigh, dorsal and inguinal) of subcutaneous adipose tissue. The proliferation rate of cASCs from abdominal and hip subcutaneous adipose tissue were higher than other depots. cASCs from abdominal subcutaneous adipose tissue had higher capacity to differentiate into adipogenic lineages than other depots. However, cASCs from dorsal subcutaneous adipose tissue had the highest capacity to differentiate into Osteogenic lineages among all depots. Regarding to the cardiomyogenic differentiation, the results showed that there was no differences among cASCs from different subcutaneous adipose tissue. Apoptosis susceptibility was demonstrated to be lowest in the superficial abdominal depot. In conclusion, proliferation, differentiate capacity and sensitivity to apoptosis of cASCs were linked to anatomic subcutaneous adipose tissue depots whereas there were no significant differences in the expression of surface antigens and gene transduction efficiency.
ER  -