TY  - JOUR
T1  - <I>In vivo</I> and <I>In vitro</I> Antibacterial Activities of Some Essential Oils of Lamiaceae Species on <I>Aeromonas salmonicida</I> Isolates from Cultured Rainbow Trout, <I>Oncorhynchus mykiss</I>
AU - Okmen, Gulten AU - Ugur, Aysel AU - Sarac, Nurdan AU - Arslan, Tulin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 15
SP  - 2762
EP  - 2768
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2762.2768
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2762.2768
KW  - Antimicrobial activity
KW  -fish pathogenic Aeromonas species
KW  -plant essential oil
KW  -immune stimulant
KW  -trout
KW  -toxic effect
AB  - In this study, usability of plant essential oils from Lamiaceae species for <I>Aeromonas salmonicida</I> infections was investigated under <I>in vitro</I> and <I>in vivo</I> conditions. A total of 18 <I>A. salmonicida</I> strains were obtained from the organs and tissues of locally cultured rainbow trout. All strains were 100% resistant to penicillin, trimethoprim and bacitrasin. All strains showed widest resistance profile to antibiotics. Antibacterial effects of essential oils obtained through water distillation of aerial parts of 4 Lamiaceae species (<I>Origanum onites</I>,<I> Origanum vulgare</I> sp. <I> hirtum</I>, <I> Thymbra spicata</I> var. <I> intricata</I> and <I> Satureja thymbra</I>) were high on 17 out of 18 strains and inhibition zones ranged between 14-25, 12-26, 10-30 and 10-33 mm, respectively but none of them was effective on <I>A. salmonicida</I> FC84 strain. The most sensitive bacteria to Origanum essential oils was<I> A. salmonicida</I> FC29 strain and the most effective essential oil was <I>S. thymbra&#146;s</I>. Therefore, MIC value of this essential oil on FC29 strain was determined. Then, the MIC value (800 &#956;g) of this essential oil were injected to experimental fishes for <I>in vivo</I> studies. Injection of the most effective dose of <I>S. thymbra</I> essential oil and its several dilutions (400, 200, 100 &#956;g &#956;L<SUP>-1</SUP>) caused toxic effect and total mortality of experimental fishes. The essential oil at the doses with low or no toxic effect did not increase the bactericidal activity of fish blood indicating that it does not protect rainbow trout against <I> A. salmonicida</I> infections. On the other hand, <I>S. thymbra</I> essential oil at non-toxic low dosages can be used as immune stimulant.
ER  -