TY  - JOUR
T1  - Coexpression of Exogenous <i>hLYZ/hLF</i> Genes in Bovine Mammary Gland Epithelial Cells
AU - Zhang, Yong AU - Cao, Jun-Wei AU - Ma, Li-Bing AU - He, Xiao-Ning AU - Bi, Cong-Ming AU - Peng, Shu-Ying AU - Hua, Song AU - Shu, Jian-Hong AU - Sai, Wu-JiaFu AU - Li, Xiang-Chen AU - Zhang, Chi AU - Zhang, Zhi-Peng 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 15
SP  - 2692
EP  - 2699
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2692.2699
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2692.2699
KW  - Human lysozyme
KW  -human lactoferrin
KW  -Green Fluorescent Protein (EGFP)
KW  -transgenic
KW  -bovine mammary gland cells
AB  - Expression sequences of human Lactoferrin (LF) and Internal 
  Ribozyme Entry Site (IRES) were successfully connected by double enzyme digestion 
  and directed cloning and recombination plasmid pIL was constructed. Mammary 
  gland specific expression plasmid pEBHIL of human lysozyme and human LF was 
  constructed by BamHI restrictive enzyme digestion. Plasmid pEBHIL was introduced 
  to cow mammary gland epithelial cells after liposome transfection. Positive 
  cells were determined by G418 and PCR after EGFP expression observed by fluorescent 
  microscope and proliferated and induced by hormone. The results of Western Blotting 
  analysis on cell supernatant showed that transfected cells had expressed and 
  secreted human lysozyme (MW 14.7 ku) and human LTF (MW 40 ku).
ER  -