TY  - JOUR
T1  - Effect of <i>Gypsum fibrosum</i> and <i>Rhizoma atractylodis</i> on <i>In 
  Vitro</i> Proliferation of Mouse Splenic Lymphocyte
AU - Chu, Yaocheng AU - Qin, Ruiling AU - Li, Xinguo AU - Liu, Fenghua AU - Xu, Jianqin AU - Liu, Fenghua AU - Xu, Jianqing 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 15
SP  - 2687
EP  - 2691
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2687.2691
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2687.2691
KW  - Qingliang electuary
KW  -Shi gao
KW  -Bai zhu
KW  -in vitro proliferation
KW  -mouse splenic lymphocyte
KW  -incubation
AB  - Qingliang electuary plays important roles in speeding up heat 
  dissipation, enhancing blood circulation and improving the body’s 
  immune system. The main ingredients of a qingliang electuary are <i>Gypsum fibrosum</i> 
  (GF) and <i>Rhizoma atractylodis</i> (RA). Here, researchers report the effect 
  of these two ingredients on <i>in vitro</i> proliferation of mouse splenic lymphocyte. 
  The extracts of GF and RA along with ConA were tested under high temperatures 
  (37, 39 and 42°C) in combination with different incubation times (1 or 4 
  h) using the MTT (3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide) 
  Method. The results showed that ConA significantly stimulated lymphocyte proliferation 
  at 37°C. Under 39 and 42°C, the stimulation diminished as incubation 
  time prolonged. High dose GF (500 &#956;g mL<sup>-1</sup>) significantly inhibited 
  proliferation under all tested conditions but low dose GF (0.05 &#956;g mL<sup>-1</sup>) 
  significantly increased lymphocyte proliferation at 42°C after 1 h incubation. 
  RA water extract at 500 &#956;g mL<sup>-1</sup> significantly increased lymphocyte 
  proliferation under all tested conditions. The effect of RA oil was weaker compared 
  to RA water extract and not consistent under different incubation conditions.
ER  -