TY  - JOUR
T1  - Individual Indentification and Parentage Verification of Thoroughbred Horses and the Korean Native Horses Based on Microsatellite Loci in Korea
AU - Cho, Gil Jae AU - Choi, Seoung Kyoon AU - Lee, Sun Young 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 15
SP  - 2647
EP  - 2651
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2647.2651
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2647.2651
KW  - Genotype
KW  -horse
KW  -microsatellite
KW  -parentage verification
KW  -products
KW  -Korea
AB  - This study was performed for individual identification and 
  parentage verification of Thoroughbred Horse (TBH) and the Korean Native Horse 
  (KNH). A total number of 1,308 (84 KNH and 1,224 TBH samples including 1,005 
  foals for parentage testing) were genotyped. Genomic DNAs were extracted from 
  whole blood or hair roots and genotyped by using 14 microsatellite markers (AHT4, 
  AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and 
  VHL20) in TBH and 17 microsatellite markers including HMS2, HTG6, HTG7 in the 
  KNH. This method consisted of multiplexing PCR procedure and showed reasonable 
  amplification of all PCR products. Genotyping were determined with an ABI 3100 
  genetic analyzer. All the loci analyzed in 17 microsatellite markers showed 
  a polymorphic patterns. The number of alleles per locus varied from 3-9 with 
  a mean value of 6.36 and 5-10 (mean 7.35) in TBH and KNH, respectively. The 
  expected heterozygosity was ranged from 0.539-0.827 (mean 0.700), from 0.387-0.841 
  (mean 0.702) in TBH and the KNH, respectively. The total exclusion probability 
  of 14 microsatellite loci was 0.9998 in TBH and 0.9999 in the KNH. Of the 14-17 
  markers, ASB2, ASB17, ASB23, HMS7, HTG10 and LEX3 loci in TBH and AHT4, AHT5, 
  CA425, HMS2, HMS3, HTG10 and LEX3 loci in the KNH have relatively high PIC value 
  (>0.7). Of the 1005 foals, 1003 foals (99.80%) were qualified by compatibility 
  according to the Mendelian fashion. These results indicated that the present 
  microsatellites serve as useful tools for individual identification and paternity 
  testing of TBH and the KNH in Korea.
ER  -