TY  - JOUR
T1  - Interaction Between VirB5 of Brucella Type IV Secretion System (TFSS) and Ferritin Heavy Polypeptide 1 (FTH1) in Murine Macrophage
AU - Chen, Chuangfu AU - Guo, Fei AU - Wang, Yuanzhi AU - Zhang, Hui AU - Qiao, Jun AU - Ren, Yan AU - Zhang, Junbo AU - Li, Zhiqiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 15
SP  - 2623
EP  - 2629
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2623.2629
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2623.2629
KW  - Yeast two-hybrid
KW  -cDNA library
KW  -FTH1
KW  -Brucella
KW  -co-immunoprecipitation
AB  - TFSS is an important virulence factor of Brucella, organized 
  as one operon containing 12 different across the cell wall bacterial proteins 
  among which VirB5 regulates the host phagocytosis of Brucella and the transportation 
  of Brucella in the host cells. This study has constructed cDNA library from 
  <I>Brucella melitensis</I> 16M-infected murine macrophage Raw264.7, identified 
  and confirmed the interaction between Brucella VirB5 and FTH1 of RAW264.7 using 
  yeast two-hybrid and Co-Immunoprecipitation (Co-IP) technologies. Subsequently, 
  the morphological changes and the expression of apoptosis-related genes in Brucella-infected 
  RAW264.7 cells have been investigated with Electron Microscope (EM) and real-time 
  quantitative RT-PCR, respectively. The present study has demonstrated that VirB5 
  and FTH1 play important roles in intracellular parasitism of Brucella and inhibition 
  of FHT1 expression accelerates the apoptosis of macrophage.
ER  -