TY  - JOUR
T1  - Application of the Dual-Luciferase Reporter Assay to the Analysis of Mouse 
  Kit and Yy1 Promoter Activation Regulation by Sohlh1
AU - Zheng, Zhihong AU - Yan, Wei AU - Luo, Guangbin AU - Du, Bing AU - Li, Shengyang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 14
SP  - 2523
EP  - 2528
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2523.2528
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2523.2528
KW  - dual-luciferase reporter assay
KW  -Yy1
KW  -Kit
KW  -Sohlh1
KW  -Mouse
KW  -China
AB  - Kit is significantly downregulated in Sohlh1 deletion ovaries and Sohlh1 was over-expressed 
in Yy1 cKO ovaries and whether Sohlh1 regulates kit and Yy1 transcription is unknown. 
Here, researchers examine the promoter transcription activation of Kit and Yy1 
regulated by Sohlh1 using dual-luciferase reporter assays. The Kit responsiveness 
of the <I>Sohlh1</I> gene has been validated and provided evidence of Sohlh1 transcriptional 
activation of the <I>Kit</I> gene promoter in the mouse ovary. In contrast, Sohlh1 
has little effect on the essential activity of Yy1 promoter. Researchers identifid 
-1769 to -297 as the transcriptional activitation region of Yy1 promoter and revealed 
strong inhibitor in -297 to +135 region where maybe G-quadruplexes motifs exist. 
Researchers found two Yy1 binding Sites in Sohlh1 promoter and surmise that Yy1 
negatively regulates Sohlh1 in mouse ovary.
ER  -