TY  - JOUR
T1  - Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>RHOA, RCHY1</I> and <I>RSU1</I>
AU - Xueshan, Xia AU - Yonggang, Liu 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 2
SP  - 252
EP  - 259
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.252.259
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.252.259
KW  - Sheep
KW  -RHOA
KW  -RCHY1
KW  -RSU1
KW  -tissue expression
KW  -China
AB  - The complete coding sequences of three sheep genes-<I>RHOA</I>,<I> RCHY1</I> and <I>RSU1</I> were amplified using the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The sheep <I>RHOA</I> gene encodes a protein of 193 amino acids that shares high homology with the ras homolog gene family, member A (RHOA) proteins of fifteen species- cattle (100%), rat (99%), human (100%), mouse (99%), chicken (99%), dog (99%), turkey (99%), sumatran orangutan (99%), poephila guttata (99%), Atlantic salmon (97%), green anole (96%), rainbow trout (96%), African clawed frog (96%), zebrafish (96%) and Western clawed frog (95%). The sheep <I>RCHY1</I> gene encodes a protein of 261 amino acids that shares high homology with the ring finger and CHY zinc finger domain containing 1 (RCHY1) proteins of nine species-cattle (94%), giant panda (92%), pig (91%), human (90%), white-tufted-ear marmoset (90%), chimpanzee (90%), rabbit (89%), mouse (86%) and rat (84%). The sheep <I>RSU1</I> gene encodes a protein of 277 amino acids that shares high homology with the ras suppressor protein 1 (RSU1) proteins of twelve species-cattle (99%), chimpanzee (98%), dog (99%), human (98%), giant panda (98%), horse (98%), rabbit (98%), chicken (96%), mouse (97%), African clawed frog (94%), Western-clawed-frog (94%) and zebrafish (91%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302078, 100302546 and 100302548. The phylogenetic analysis revealed that the sheep <I>RHOA</I>, <I>RCHY1</I> and <I>RSU1</I> genes all have a closer genetic relationship with the <I>RHOA</I>, <I>RCHY1</I> and <I>RSU1</I> genes of cattle. Tissue expression profile analysis demonstrated that sheep <I>RHOA</I>, <I>RCHY1</I> and <I>RSU1</I> genes were all generally but differentially expressed in detected tissues.
ER  -