TY  - JOUR
T1  - Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I>
AU - Xueshan, Xia AU - Yonggang, Liu 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 2
SP  - 245
EP  - 251
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.245.251
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.245.251
KW  - Sheep
KW  -ZFAND5
KW  -ZGPAT
KW  -ZDHHC7
KW  -tissue expression
KW  -China
AB  - The complete coding sequences of three sheep genes <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The sheep <I>ZFAND5</I> gene encodes a protein of 213 amino acids that shares high homology with the Zinc finger, AN1-type Domain 5 (ZFAND5) proteins of eight species; cattle (100%), pig (99%), human (99%), rabbit (99%), mouse (98%), giant panda (98%), gray short-tailed opossum (97%) and Northern white-cheeked gibbon (97%). The sheep <I>ZGPAT</I> gene encodes a protein of 513 amino acids that shares high homology with the Zinc finger CCCH-type with G patch domain-containing protein (ZGPAT) proteins of seven species; cattle (97%), giant panda (84%), rabbit (99%), human (79%), rat (76%), mouse (77%) and chimpanzee (78%). The sheep <I>ZDHHC7</I> gene encodes a protein of 308 amino acids that shares high homology with the Zinc finger, DHHC-type containing 7 (ZDHHC7) proteins of nine species; cattle (99%), dog (93%), pig (93%), human (93%), giant panda (93%), Northern white-cheeked gibbon (92%), mouse (92%), white-tufted-ear marmoset (92%) and rat (92%). Finally, these three novel sheep genes were assigned to GeneIDs; 100302558, 100302028 and 100302557. The phylogenetic analysis revealed that the sheep <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> genes all have a closer genetic relationship with the <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> genes of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> genes were all generally but differentially expressed in detected tissues.
ER  -