TY  - JOUR
T1  - Amplification of Nucleoprotein (<I>NP</I>) Gene from Canine Influenza Virus 
  H3N2 and its Expression in <I>Escherichia coli</I>
AU - Shou-Jun, Li AU - Li, Wang AU - Yu-Fei, Guo AU - Hai-Tao, Qi AU - Shuo, Su AU - Ji-Dang, Chen AU - Fu-Rong, Zhao AU - Nan, Cao AU - Min-Ze, Zhang AU - Chao-Yi, Zhang AU - Zhen, Huang AU - Li-Kai, Tan AU - Gui-Hong, Zhang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 14
SP  - 2432
EP  - 2435
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2432.2435
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2432.2435
KW  - Canine influenza virus
KW  -NP gene
KW  -cloning
KW  -gene expression
KW  -protein
AB  - NP is a relatively conservative structure protein also is the main recognition antigen and have good middle cross reactive. To express Canine Influenza Virus (CIV) subtype H3N2 NP protein, the <I>NP</I> gene was amplified by PCR and cloned into vector pMD18-T and then sequence determination. The <I>NP</I> gene was cloned into vector pET-32a (+). The recombinant plasmid pET-NP was transformed into Rosetta II (DE3) and induced with IPTG.
ER  -