TY  - JOUR
T1  - An Improved Reverse Transcription Loop-Mediated Isothermal Amplification Assay 
  for Sensitive and Specific Detection of Infectious Bronchitis Virus
AU - Cao, Yongchang AU - Luo, Hongbin AU - Zhu, Daozhong AU - Xue, Chunyi AU - Qin, Jianping AU - Chen, Feng 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 14
SP  - 2398
EP  - 2402
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2398.2402
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2398.2402
KW  - Infectious bronchitis virus
KW  -loop-mediated isothermal amplification
KW  -IBV strains
KW  -RT-LAMP
AB  - A highly conserved region of <I>1a </I>gene of Infectious Bronchitis Virus (IBV) was chosen to design the Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) degenerate primers. The developed RT-LAMP assay is a highly sensitive, specific, rapid method to detect IBV in allantoid and tissue. Optimal temperature was around 62&deg;C and duration was 45 min. Sensitivity analysis showed that RT-LAMP assay was 10 fold more sensitive than RT-PCR. Specificity analysis showed that 3 IBV strains omitted by the previous reported RT-LAMP assay could be detected by the improved RT-LAMP assay. In field trials, the improved RT-LAMP assay obtained 98.4% sensitivity in 65 clinical samples while 95.4% of RT-PCR and 93.8% of the previous reported RT-LAMP which indicates it is a powerful tool in the practical application.
ER  -