TY  - JOUR
T1  - Generate Induced Pluripotent Stem Cells by Three Factors under Feeder-Free Condition with Higher Efficiencies
AU - Wang, Huanhuan AU - Song, Chunlei AU - Dong, Fulu AU - Lu, Youde AU - Du, Changwan AU - Han, Jing AU - Qin, Ling AU - Zhai, Le AU - Zhao, Fang AU - Xie, Zhourui AU - Liu, Honglin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 12
SP  - 2110
EP  - 2115
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2110.2115
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2110.2115
KW  - Induced Pluripotent Stem (iPS) cells
KW  -MEF
KW  -feeder
KW  -feeder-free
KW  -reprogramming
AB  - Pluripotent stem cells can be induced from different cell types through transduction of the transcription factors by lentiviral, polycistronic vectors and other vectors. Most of these methods of generation of induced Pluripotent Stem (iPS) cells still use Mouse Embryonic Fibroblasts (MEF) as feeder layers. The use of animal feeder layers will hinder the clinical application of iPS cells because of the presence of unknown animal products, xenobiotics and when screening the intermediates iPS to study the mechanisms of cellular reprogramming, the feeder cells would be mixed in intermediates iPS cells samples. Here researchers generated iPS cells from MEF use only three factors Oct4, Klf4 and Sox2. Through add Vitamin C and gradually replaced the medium after retroviral infection, researchers promote the efficiency of induced iPS to 0.2%.
ER  -