TY  - JOUR
T1  - Construction of Full-Length Goose Muscle cDNA Library
AU - Wu, Wei AU - Chen, Lu AU - Zhu, Haiyang AU - Sui, Yujian AU - Fu, Yingying AU - Hao, Zhe AU - Hu, Jingtao AU - Yang, Tongao AU - Sun, Yongfeng 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 12
SP  - 2106
EP  - 2109
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2106.2109
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2106.2109
KW  - China
KW  -PCR amplification
KW  -SMART technique
KW  -cDNA library
KW  -Goose
AB  - The muscular tissue of breast was dissected from 8 weeks old Jilin White goose in the present study. The big fragment PCR Method was used to amplify double-strand cDNA based on the SMART techniques for construction of a full-length cDNA library. After digestion with restriction endonuclease Sfi &#124;, a modified vector of pBluescript II SK-plasmid with the adaptors containing Sfi &#124;A and Sfi &#124;B sites was used to recombine with the cDNA products amplified. The recombinants were cloned by transformation into competent <I>Escherichia coli</I> DH2&#945;. A plasmid cDNA library with goose muscle was constructed. The results showed that the titer of the cDNA library was 1.01x10<SUP>6</SUP> pfu mL<SUP>-1</SUP> and the percentage of recombinant clones was 97%. The length of most cDNA inserted was between 0.25 and 1.6 kb identified by gel electrophoresis after cDNA PCR amplification. The unigene ratio was 66.7% and the percentage of complete cDNA sequences was 80% by estimating from the 24 clones sequenced randomly. It is helpful to study muscle development of goose at molecular level in the future.
ER  -