TY  - JOUR
T1  - Transgenic Expression and Antimicrobial Potential of CAMA-Syn in A549 Cells
AU - Wang, Huayan AU - Zhang, Junlin AU - Wei, Yulei AU - Qin, Mingming AU - Yang, Fan 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 12
SP  - 2006
EP  - 2012
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2006.2012
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2006.2012
KW  - CAMA-syn
KW  -lung epithelium
KW  -antibacterial assay
KW  -antimicrobial peptide
KW  -N-terminal
KW  -China
AB  - CAMA-syn, a hybrid composed of N-terminal &#945;-helical segment of CA (amino acid 1-8) and N-terminal &#945;-helical segment of MA (amino acid 1-12) is a novel small peptide with the potent antibacterial and synergistic activity without cytotoxicity. In order to test the antibacterial function of CAMA-syn produced specially in lung epitheliuml cells, several vectors containing the synthesized CAMA-syn DNA fragment initiated by CMV and Surfactant Protein C (SP-C) promoter were constructed and transfected into recipient cells. The results showed that CAMA-syn fusion to Green Fluorescent Protein (GFP) or to Hemagglutinin epitope (HA) tag was expressed in both 293 and A549 cells. The antibacterial assays of CAMA-syn were conducted against both Gram positive and negative bacteria including <I>S. abortusovis</I>, <I>P. anatis</I>, <I>S. hyicus</I> and <I>S. suis</I>. The results of colony forming efficiency and cell growth curves proved that the <I>in vitro</I> expressed CAMA-syn could inhibit the growth of bacterial, demonstrating that lung epithelial cell-specific expression of antimicrobial peptide CAMA-syn could have antibacterial activity.
ER  -