TY  - JOUR
T1  - Analysis of Secreted Proteins from <I>Undifilum cinereum</I> by Two Dimensional Gel Electrophoresis and Liquid Chromatography-Mass Spectrometry/Mass Spectrometry
AU - Yu, Yuanying AU - Li, Haili AU - Gao, Rui AU - Baucom, Deanna AU - Creamer, Rebecca AU - Yang, Zijun AU - Wang, Jianhua AU - Yang, Guodong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1881
EP  - 1889
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1881.1889
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1881.1889
KW  - mass spectrometry
KW  -proteomics
KW  -Undifilum cinereum fungus
KW  -2-DE
KW  -swainsonine
KW  -astragalus
KW  -locoweed
AB  - The locoweed plant (Astragalus) is a widely distributed toxic plant in many rangeland regions around the world. It is well known that locoweed plants can produce the alkaloid swainsonine which inhibits &#945;-mannosidases and causing neurological poisonings problems through the consumption of locoweed. Locoweed poisoned grazing animal&#146;s exhibit symptoms of locoism. Locoism was caused by locoweed is one of the most destructive disease of rangeland. Recent studies shown that swainsonine was produced by endophytic <I>Undifilum cinereum</I> which was isolated from Astragalus locoweed (<I>Astragalus mollissimus</I> and <I>Astragalus lentiginosus</I> sp.) and responsible for locoism in grazing animals. The toxicosis effect of <I>U. oxytropis</I> fungi on rats is indistinguishable from locoweed toxicosis on rats. The mechanisms of swainsonine underlying <I>U. cinereum</I> and locoweed are poorly understood. To gain a better understanding of the swainsonine biosynthesis in <I>U. cinereum</I> and to facilitate management of locoweed poisoning problems, two-dimensional gel electrophoresis (2-DE) was performed. The 2-DE is a promising tool to study the protein expression profiling and metabolic pathway. To researchers knowledge the present study was the first proteomic reference map using immobilized pH gradients of <I>U. cinereum</I>. To identify proteins in <I>U. cinereum</I>, proteins extracted from mycelial were separated by 2-DE and IEF, digestion and Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) with an LTQ ion trap mass spectrometer (Thermo Scientific, Waltham, MA). Samples were analyzed by LC-MS/MS and identified using MASCOT MS/MS search in protein databases.
ER  -