TY  - JOUR
T1  - Comparison Between the Effects of Valproic Acid and Trichostatin A on <I>in vitro</I> Development of Sheep Somatic Cell Nuclear Transfer Embryos
AU - Chen, Chuangfu AU - Meng, Ren AU - Hazi, Wureli AU - Hu, Shengwei AU - Ni, Wei AU - He, Zhirui AU - Sai, Wujiafu AU - Guo, Jixing 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1868
EP  - 1872
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1868.1872
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1868.1872
KW  - development-related genes
KW  -Valproic acid
KW  -SCNT embryo
KW  -Trichostatin A
KW  -sheep
AB  - The present study was carried out to assess and compare the effects of Valproic Acid (VPA) and Trichostatin A (TSA) on <I>in vitro</I> development of sheep Somatic Cell Nuclear Transfer (SCNT) embryos. The results showed that treatment of cloned sheep embryos with 4 mM VPA or 50 nM TSA for 24 h after activation could significantly improve blastocyst rate compared to the control (30.7 vs. 23.3 vs. 16.7%, respectively p&lt;0.05). VPA treatment resulted in a significant higher blastocyst rate than that of TSA-treated group (p&lt;0.05). Moreover, VPA treatment significantly increased (p&lt;0.05) total cell number per blastocyst compared with the TSA treatment and control groups (78.8&plusmn;9.3 vs. 69.6&plusmn;9.7 vs. 64.1&plusmn;8.6, respectively). Furthermore, VPA treatment increased expression of the development-related genes OCT4 and SOX2 in SCNT blastocysts. These results demonstrate that VPA may be more potent than TSA in supporting developmental competence of cloned embryos.
ER  -