TY  - JOUR
T1  - Study of a Simple and Rapid PCR Sex Identification of Bovine Embryo
AU - Wang, Dong AU - Liu, Yan AU - Qin, Tong AU - Du, Weihua AU - Li, Shujing AU - Zhao, Xueming AU - Chen, Jun AU - Hao, Haisheng AU - Lin, Bo AU - Zhu, Huabin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1847
EP  - 1852
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1847.1852
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1847.1852
KW  - sex chromosome
KW  -simple PCR
KW  -sex identification
KW  -rapid PCR
KW  -Bovine embryo
KW  -China
AB  - A rapid and simple PCR sex identification of embryo is very important for bovine embryo transferring. Many sex identification methods using duplex PCR were established according to <I>Sry</I> gene. But the identification process was affected greatly by more primers interaction. In order to decrease the interference from more primers, researchers explored a simple and rapid PCR Method. The sequences of Amelogenin alleles located at both sex chromosomes were downloaded from GenBank. A pair of sex specific primers was designed to span the 63 bp longer insertion sequence in X chromosome. Bovine samples of blood, fibroblasts and demi-embryos were sexed with these primers. Two-temperature PCR cycling program was used in which the extension step was deleted while the denaturizing and annealing steps were shortened to 1 sec. The results shown ideal identification were obtained and observable amplification were also obtained using even single fibroblast. About 20 bovine embryos were identified by this PCR cycling program and 15 embryos (9 females and 6 males) were transferred. The sexing results were confirmed by the anatomically proven sex after parturition, respectively. The comparison of amplification results between blood samples of bovine and human shows the excellent, specificity to bovine. Thus, a simple, rapid and effective PCR sex identification method was established.
ER  -