TY  - JOUR
T1  - Determination of Digestive Enzyme Activity in the Digesta from the Small Intestinal of Growing Pigs and Development of <I>in vitro</I> Evaluation System for Feed Bioavailability Using Artificial Small Intestinal Juice
AU - Li, Defa AU - Wang, Junjun AU - Zhao, Feng AU - Zhang, Hongfu AU - Fang, Luoyun 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1819
EP  - 1826
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1819.1826
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1819.1826
KW  - Digestive enzyme activity
KW  -growing pig
KW  -in vitro
KW  -feed evaluation
KW  -intestinal fluid
KW  -China
AB  - The objective of this study was to determine enzyme activity in the digesta from the small intestine of growing pigs and develope an <I>in vitro</I> evaluation system for feed bioavailability using artificial small intestinal juice. Ten pigs (22.77&plusmn;0.89 kg and fitted with a simple T-cannula at the jejunum) were used in a doubly 5x5 Latin square design. In each period, pigs were offered one of five diets differing in nutrient level for 14 days. The Standard diet (ST) contained 3400 kcal kg<SUP>-1</SUP> of Digestible Energy value (DE) and 17% Crude Protein (CP). The DE for the other four diets was 3600, 3200, 3600 and 3200 kcal kg<SUP>-1</SUP>, respectively while the CP level was 21, 13, 13 and 21%, respectively. The small intestinal digesta was collected for determining digestive enzyme activity. Meanwhile, feces were collected for determining <I>in vivo</I> feed digestibility. The results showed that the range of amylase, trypsin, chymotrypsin and lipase activities in the intestinal fluid of growing pigs was 15.52-251.43, 21.24-67.39, 3.45-19.17 and 0.02-3.59 U mL<SUP>-1</SUP>, respectively. To establish an <I>in vitro</I> evaluation system for feed bioavailability, Artificial Small Intestinal Juice (ASIJ) was prepared with mixed enzyme reagents based on the mean activities of amylase, trypsin, chymotrypsin and lipase in the digesta used to evaluate the five diets by three-stage enzymatic incubation. By comparing the DE of the five feedstuffs from the <I>in vivo </I>method and the digestibility of the feedstuffs from the<I> in vitro</I> ASIJ analysis, mathematical models for predicting <I>in vivo</I> DE of<I> In Vitro</I> Dry Matter (IVDM), Organic Matter (IVOM) and DE (IVDE) was established (DE = 0.1076xIVDM+0.3741, R<SUP>2</SUP> = 0.34; DE = 0.1276xIVOM+1.6486, R<SUP>2</SUP> = 0.31; DE = 0.4625xIVDE+7.2065, R<SUP>2</SUP> = 0.71). There were no significant differences between the <I>in vitro</I> evaluation results and the developed <I>in vitro</I> method. Therefore, the system in this study based on ASIJ is a convinent and reasonably accurate method for <I>in vitro</I> evaluation of feed bioavailability.
ER  -