TY  - JOUR
T1  - Trichostatin A Treatment on Two Types of Donor Cells for Somatic Cell Nuclear Transfer
AU - Li, He-Ping AU - Yao, Ya-Xin AU - Su, Xiao-Hua AU - Ma, Yue-Hui AU - Li, Xiang-Chen AU - Guan, Wei-Jun 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1812
EP  - 1818
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1812.1818
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1812.1818
KW  - TSA
KW  -histone acetylation
KW  -donor cell
KW  -reprogramming
KW  -IVF
KW  -China
AB  - An increased histone acetylation is associated with more effective formation of DNA replication complexes (Trichostatin A, TSA), a histone deacetylase inhibitor is able to enhance the pool of acetylated histone. Researchers observed the changes of the Sheep Fibroblasts Cells (SFCs) and Sheep Cumulus Cells (SCCs) being treated with TSA, a stronger immunofluorescent signal for acH4K12 was detected in the 1 cell stage (p&lt;0.05) also the developmental rates of cloned embryos was increased significantly (p&lt;0.05) however, a higher acetylation level were observed in SCCs naturely, no significant effects on acetylation level and developmental rates of cloned embryos were detected after TSA treatment. Moreover, the acetylation distribution in the group of SFC-TSA cloned embryos resembles that in <I>In Vitro</I> Fertilisation (IVF) embryos but TSA had no ability to modify the acetylation state of SCCs cloned embryos similar with the IVF embryos, only a hyperacetylate pattern was detected which means a proper pattern of acetylation in cloned embryo is more prone to be reprogrammed than hyperacetylation.
ER  -