TY  - JOUR
T1  - Polymorphism Analysis on Intron 3 of the <I>GH</I> Gene in Chinese Donkey
AU - Su, Yongmei AU - Zhu, Wenjin AU - Wu, Jianhua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 10
SP  - 1705
EP  - 1707
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1705.1707
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1705.1707
KW  - Donkey
KW  -GH gene
KW  -intron 3
KW  -polymorphism
KW  -PCR-SSCP
KW  -China
AB  - This experiment was conducted to study the polymorphism on intron 3 of <I>GH</I> gene in donkey in China. The polymorphism was analysised by PCR-SSCP in the 10 donkey breeds (JN, GL, GZ, DZ, JM, BY, QY, LZ, TH and XJ) by PCR-SSCP. One SNPs was found in the sequence of <I>GH</I> gene through the sequencing analysis of the two homozygous genes <I>AA</I> and <I>BB</I>, the mutation at 100th site (G&rarr;T) of the third intron of donkey <I>GH</I> gene was showed. Three genotypes AA, AB and BB were detected in 10 donkey breeds and the allele A was the predominant allele. All the breeds were in Hardy-Weinberg equilibrium at this polymorphic site (p&gt;0.05). The results confirmed that there were polymorphisms in the intron 3 of <I>GH</I> gene.
ER  -