TY  - JOUR
T1  - A Simple Cultural Method for Detection of <I>Mycoplasma bovis</I>
AU - Hong, Nining AU - De-Xing, Qian AU - Deng-Xiang, Zhang AU - Long-Zhong, Ran 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 10
SP  - 1643
EP  - 1646
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1643.1646
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1643.1646
KW  - Culture
KW  -detect
KW  -method
KW  -Mycoplasma bovis
KW  -media
KW  -CO2
AB  - Simple method for the detection of <I>Mycoplasma bovis</I> in both liquid and solid cultures was established. Fresh media were easily prepared and dispatched into an eppendorf tube or a Petri dish, a new cultural procedure was followed with the eppendorf tube closed or the Petri dish sealed. Compared to the conventional methods, appropriate inoculating and subculturing operations got easier and a CO<SUB>2</SUB> incubator was not required; moreover, probable contamination by airborne fungi or bacteria could be reduced or eliminated during long-term incubation. Similarly, <I>M. bovis</I> grew normally and could be investigated by Color-Change Units (CCU) enumeration, colony morphology or PCR assay.
ER  -