TY  - JOUR
T1  - Immunogenicity of Foot and Mouth Disease Virus Type Asia 1 Protein VP1-2A Fused with a Multi-Epitope Expressed in <I>Pichia pastoris</I>
AU - , Ningyi Jin AU - Zhu, Zhan-Bo AU - , Ying Xi AU - , Bo Hu AU - , Chang Li AU - , Huijun Lu AU - , Kuoshi Jin AU - , Jingqiang Ren AU - , Cunxia Liu AU - , Minglan Jin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 9
SP  - 1512
EP  - 1517
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1512.1517
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1512.1517
KW  - FMDV
KW  -VP1-2A
KW  -CTE
KW  -Pichia pastoris
KW  -immunogenicity
KW  -China
AB  - Foot and Mouth Disease (FMD) is a highly infectious and economically devastating disease of livestock. A genetically engineered vaccine against Foot and Mouth Disease Virus (FMDV) may be a good control measure for use during an epidemic. In this study, a secretory expression vector, pPIC9K-VP1-2A-CTE, containing a fusion of the bovine (FMDV) type Asia 1 <I>VP1-2A </I>gene and a multi-epitope CTB-TEpi (CTE) was constructed. After being linearized with Sal I, the recombinant plasmid was transformed into <I>Pichia pastoris</I> GS115 by electroporation. The fusion protein expressed in yeast was analyzed by SDS-PAGE and Western blot and immunogenicity of the fusion protein was assessed. The expressed recombinant VP1-2A and CTE protein elicited specific humoral and cellular immune responses in mice. Additionally, immunized guinea pigs were protected when challenged with the FMD Asia 1 virus.
ER  -