TY  - JOUR
T1  - Effect of n-3 Polyunsaturated Fatty Acids on Induction of Hypoxia Inducible Factor-2 Alpha in C<SUB>2</SUB>C<SUB>12</SUB> Myotubes
AU - , Defa Li AU - Zhang, Jize AU - , Xiangshu Piao 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 9
SP  - 1411
EP  - 1419
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1411.1419
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1411.1419
KW  - n-3 PUFAs
KW  -HIF-2a
KW  -PDK4
KW  -PPARa
KW  -basal metabolism
KW  -skeletal muscle
AB  - Results from different trails have provided evidence of protective effects of n-3 Polyunsaturated Fatty Acids (n-3 PUFAs) on heart and skeletal muscle. But the induction of Hypoxia Inducible Factors (HIFs) by n-3 PUFAs in these protective effects has never been reported before. The aim of this study was to find out if the marine n-3 fatty acids Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) and of the plant-derived Alpha Linolenic Acid (ALA) and Stearidonic Acid (SDA) can induce HIFs in C<SUB>2</SUB>C<SUB>12</SUB> myotubes and subsequent effect on cell metabolism. The n-3 PUFAs, especially DHA, EPA and SDA increased the levels of protein and mRNA in HIF-2&#945; at 72 h. Meanwhile, n-3 PUFAs also elevated the expression of HIF related transcriptional factors like PDK4 and PPAR&#945; at 72 h. The reprogramming of basal metabolism in C<SUB>2</SUB>C<SUB>12</SUB> myotubes increased in <I>GLUT4</I> and <I>CPT1b</I> gene expression by treatment of n-3 PUFAs at 72 h. UCP2 was increased by n-3 PUFAs for attenuating production of ROS at 72 h. n-3 PUFAs, especially DHA, EPA and SDA could induce HIF-2&#945; which is associated with upregulation of PDK4 by activation of PPAR&#945;. This process also implies a reprogramming of basal metabolism and oxidative damage protection in C<SUB>2</SUB>C<SUB>12</SUB> myotubes.
ER  -