TY  - JOUR
T1  - Porcine <I>RPL28</I> Gene, Differentially Expressed in the Backfat Tissue Between Meishan, Large White Pigs and Their F1 Hybrids
AU - Ren, Z.Q. AU - , Y.Z. Xiong AU - , W.J. Wu AU - , C.Y. Deng AU - , S.W. Jiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 8
SP  - 1237
EP  - 1241
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1237.1241
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1237.1241
KW  - RPL28
KW  -differential expression
KW  -pig
KW  -heterosis
KW  -backfat
KW  -China
AB  - The mRNA differential display technique was performed to investigate the differences in gene expression in the backfat tissues between Meishan, Large White pigs and their F1 hybrids. One novel gene that was differentially expressed was identified through both semi-quantitative Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and Quantitative reverse transcriptase Polymerase Chain Reaction (Q-PCR) and it was highly expressed in F1 hybirds<SPAN LANG="EN-GB">, Meishan x Large White and Large White x Meishan compared to their parents. </SPAN>The full-length cDNA was then cloned using the Rapid Amplification of cDNA Ends (RACE) Method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encods a protein of 137 amino acids has high homology with the RPL 28 of seven species-cattle (97%), human (97%), mouse (95%) and rat (97%) so that it can be defined as the porcine <I>RPL28</I> gene. Tissue expression profile analysis indicated that the swine <I>RPL28</I> gene is differentially expressed in detected tissues. The experiment suggested that the swine <I>RPL28</I> gene might play role in the formation of heterosis.
ER  -