TY  - JOUR
T1  - Construction of RNAi Expressing Vector Targeting the Goat Prion Protein Gene (<I>PRNP</I>)
AU - Li, Yongjun AU - , Wenting Li AU - , Taikun Li AU - , Guangjing Zhang AU - , Jiahui Zhang AU - , Dejun Ji 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 8
SP  - 1208
EP  - 1212
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1208.1212
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1208.1212
KW  - RNA interference
KW  -siRNA
KW  -prion protein gene
KW  -goat
KW  -antagonistic
KW  -protein
AB  - To explore the effect of RNA interference on the suppression of goat prion protein gene (<I>PRNP</I>), small interfering RNA (siRNA) expressing vectors pSilencer4.1-siRNA1 and a negative control pSilencer4.1-siRNA2 and the eukaryotic expressing vector pSG5-prion expressing parts of goat prion protein gene were designed and constructed. The recombinants were introduced into Human Embryonic Kidney (HEK) 293T cells mediated by GeneTran. RT-PCR was carried out to detect the expression of prion protein gene. The results showed that RNAi effects induced by pSilencer4.1-siRNA1 could successfully deplete the mRNA expression of goat prion protein gene in 293T cells 48 h after transfection with both vectors compared to the negative control. The findings demonstrated that pSilencer4.1-siRNA1 was constructed successfully to transfer antagonistic substance against goat prion protein gene.
ER  -