TY  - JOUR
T1  - Molecullar Typing, Prevalence of netB and Antimicrobial Susceptibility among Clinical Isolates of <I>Clostridium perfringens</I> from Chickens in Southern China
AU - , Mingfei Sun AU - Wu, Caiyan AU - , Shenquan Liao AU - , Nanshan Qi AU - , Xinyu Peng, AU - , Minna Lv 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 8
SP  - 1183
EP  - 1187
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1183.1187
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1183.1187
KW  - China
KW  -Clostridium perfringens
KW  -molecullar typing
KW  -antimicrobial susceptibility
KW  -isolates
KW  -genes
AB  - <I>Clostridium perfringens </I>is an important pathogen of animals and humans and is the causative agent of Necrotic Enteritis (NE) in poultry. This study focused on the typing of intestinal <I> C. perfringens</I> isolates (n = 78, 54 from chickens with NE, 24 from healthy chickens) from China&#146;s Southern Guangdong province. All isolates were classified as type A using multiplex PCR assay. The recently described toxin gene associated with NE in chickens, netB was found in 21 (26.9%) of the isolates (18 from chickens with NE and 3 from unrelated chickens with no evidence of NE). The <I>in vitro</I> susceptibility results showed that ceftriaxone showed highest activity with lowest MIC<SUB>50</SUB> values (0.25-0.5 &#956;g mL<SUP>-1</SUP>) followed by cefotaxime (0.25-4 &#956;g mL<SUP>-1</SUP>), penicilin (0.5-2 &#956;g mL<SUP>-1</SUP>). However, most of the isolates were resistant to lincomycin, tetracycline, metronidazole and neomycin as evident by the high MIC<SUB>50</SUB>. Furthermore, 70.5% of the isolates (n = 55) were simultaneously resistant to &gt;4 antibiotics and 30.8% of the isolates (n = 24) were resistant to all the antimicrobials tested. To the knowledge this is the first study of the prevalence of <I>C. perfringens </I>in broiler chickens in China and the first to examine the isolates for the recently identified novel toxin genes, <I>netB</I>.
ER  -