TY  - JOUR
T1  - Cloning and Expression of Transforming Growth Factor Beta 1 of Guanzhong Dairy Goat and its Effect on Proliferation of Goat Peripheral Blood Mononuclear Cells (PBMCs)
AU - , D.K. Chen AU - Shang, C.C. AU - , Y.Q. Zhao AU - , D.Y. Cao AU - , Y.L. Yao AU - , J. Luo 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 8
SP  - 1127
EP  - 1134
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1127.1134
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1127.1134
KW  - TGF-B1
KW  -PBMCs
KW  -proliferation
KW  -dairy goat
KW  -physiological state
KW  -amino acid
AB  - Transforming Growth Factor beta 1 (TGF-&#946;1) is a pleiotropic cytokine that affects diverse biologic processes. In this study, the goat <I>TGF-&#946;1</I> gene was cloned and sequenced with length of 1173 bp. The predicted peptide of goat TGF-&#946;1 contained 390 amino acids and 29 amino acids signal peptide. Phylogenetic analysis of different species available in GenBank<SUP>TM</SUP> based on the complete amino acid sequences of TGF-&#946;1 showed that the goat was clustered with sheep sistered to bovine. The TGF-&#946;1 mature peptide including 112 amino acids was expressed with a fusion protein of approximately 32 kDa. The goat PBMCs were proliferative stimulated by recombinant TGF-&#946;1 but the proliferation of PBMCs stimulated by ConA was inhibited under co-stimulation of recombinant TGF-&#946;1. These results suggest that the goat recombinant TGF-&#946;1 would play an important role in regulating the immune responses and sustaining the balance of organism physiological state.
ER  -