TY  - JOUR
T1  - Prokaryotic Expression and Sequence Analysis of Porcine <I>BCL10</I> Gene
AU - Guo, Wanzhu AU - Zuo, Zhicai AU - Liao, Shan AU - Qi, Xinqiao AU - Yi, Yue AU - Xu, Zhiwen AU - Zhu, Ling AU - Cui, Hengmin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 7
SP  - 1046
EP  - 1051
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1046.1051
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1046.1051
KW  - BCL-10
KW  -molecular cloning
KW  -Bioinformatics
KW  -Prokaryotic expression
KW  -human
KW  -China
AB  - BCL-10 is an important functional and an apoptotic regulator gene inducing the organismic innate immunity. It has not been reported that the BCL-10 protein of porcine in normal level could motivate immune factor to produce. Bioinformatics analysis was performed to predict the characteristics of BCL-10, the results indicated that <I>BCL-10</I> gene encoded a polypeptide, molecular mass of 45 Ku and comprising 233 amino acids. The prediction of secondary structure showed that BCL-10 had 9 alpha-helix, 1 beta-sheet, 12 turn and 14 random coil. The protein had twenty transmembrane regions, an CARD structure field which located in 15-100 amino acids and no signal peptide, the hydrophobic index was between -2.500 and 1.400. The phylogenetic tree showed that BCL-10 was similar to human (the homology index was &gt;95%) and had a distant relationship to mouse. To identify the activity of BCL-10, the protein with a His-tag was expressed in <I>E. coli</I> Rosetta and purified. The purified protein showed an obvious effect of cell proliferation. Cloning and analysising of <I>BCL-10</I> gene which laid foundation to further research and exploitation.
ER  -