TY  - JOUR
T1  - Isolation and Identification of <I> Bucella melitensis</I> in Goats
AU - Omar, A.R. AU - AL-Garadi, Maged Ahmed AU - Khairani-Bejo, S. AU - Zunita, Z. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 8
SP  - 972
EP  - 979
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.972.979
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.972.979
KW  - Goat
KW  -Brucellosis
KW  -isolation
KW  -Real-time PCR
KW  -PCR
KW  -goats
AB  - Isolation of <I>Brucella melitensis</I> is the standard gold of identification and confirmation of animal brucellosis. However in Malaysia, <I>Brucella</I> sp., infection of goat was increasing recently and there is no evidence for diagnosis of the serover of <I>Brucella</I> sp., that cause the disease in goat population except the detection of serological methods. Isolation and identification of <I>Brucella melitensis</I> have been done by bacteriological methods in addition to conventional Polymerase Chain Reaction (PCR) and Real-time PCR for detection of <I>Brucella melitensis</I> from samples collected from vaginal swabs on suspected farm. In conclusion, four isolate have been got out of 300 vaginal samples and all isolate is belong to <I>Brucella melitensis</I> server 1. The Real-time PCR is the easy and save method for confirmation of brucellosis in goats population.
ER  -