TY  - JOUR
T1  - Populations of <I>Salmonella enteritidis</I> in the Tissues of Reproductive Organs in Laying Hens After Oral Challenge: A Real-Time PCR Study
AU - He, Guang-Zhi AU - Tian, Wei-Yi AU - Qian, Ning AU - Deng, Shu-Xuan 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 8
SP  - 945
EP  - 948
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.945.948
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.945.948
KW  - Salmonella enteritidis
KW  -reproductive organs
KW  -population
KW  -vagina
KW  -concentration
KW  -China
AB  - The objective of this study was to determine the populations of <I> Salmonella enteritidis</I> (<I>S. enteritidis)</I> in reproductive organs of laying hens after oral challenge. Researchers conducted serovar-specific Real-time PCR for <I>S. enteritidis</I> to detect the genomic DNA of <I>S. enteritidis</I> from laying hens at different time points. To validate these results, the Indirect Fluorescent Antibody (IFA) technique was employed too. The results showed that <I>S. enteritidis</I> was consistently detected in all the samples. Vagina and uterus were positive at 20 h PI and the last organ to show a positive result was the largest and third largest preovulatory follicle at 32 h PI. The copy numbers of <I>S. enteritidis</I> DNA in each tissue reached a peak at 36-60 h PI with the vagina and uterus containing higher concentrations than other tissues. However, the number of bacteria started decreasing by 3-4 days and by 6 days, the concentration of <I>S. enteritidis</I> DNA was below the detection limits of the PCR assay except the vagina. In conclusion, the results provided insights into the <I>S. enteritidis</I> populations in the reproductive organs. This study will help in understanding the pathogenesis of <I>S. enteritidis</I> infection <I>in vivo</I>.
ER  -