TY  - JOUR
T1  - Culture of Chicken Embryonic Stem Cells in Buffalo Rat Liver 3A Cells (BRL-3A) Conditioned Medium
AU - Jia, Qing-Hui AU - Fu, Zhi-Xin AU - Juan, Chen AU - Liu, Zheng-Zhu AU - Ping, Shen AU - DU, Li-Xin AU - Zhang, Chuan-Sheng AU - Liu, Xie-Rong AU - Geng, Li-Ying 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 6
SP  - 791
EP  - 795
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.791.795
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.791.795
KW  - cell clones
KW  -BRL-3A cells
KW  -in vitro culture
KW  -ES cell
KW  -Chicken
KW  -embryonic stem
AB  - BRL-3A conditioned medium combined with basic Fibroblast Growth Factor (bFGF), Insulin-like Growth Factor (IGF), Stem Cell Factor (SCF) and STO cells are used for the culture of the feeder layer and ES (Embryonic Stem) cell clones. This study is done by isolation of chicken X-stage blastodermal cells. The putative ES cell clone features a typical bird&#146;s nest structure, PAS and AKP-positive and strongly positive for SSEA-1. It is able to form embryoid bodies and multiple types of cells as well as participates in embryonic development and feather color chimera formation through <I>in vitro</I> differentiation.
ER  -