TY  - JOUR
T1  - Characterisation of <I>Angiostrongylus cantonensis</I> Isolates from China by Sequences of Internal Transcribed Spacers of Nuclear Ribosomal DNA
AU - Lin, R.Q. AU - Zhu, X.Q. AU - Wu, C.Y. AU - Chen, M.X. AU - Ai, L. AU - Li, J. AU - Zhang, R.L. AU - Liu, C.Y. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 5
SP  - 593
EP  - 596
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.593.596
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.593.596
KW  - China
KW  -sequence analysis
KW  -Polymerase Chain Reaction (PCR)
KW  -Angiostrongylus cantonensis
KW  -Internal Transcribed Spacer (ITS)
KW  -ribosomal DNA (rDNA)
AB  - The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) among <I>Angiostrongylus cantonensis </I>isolates from Shenzhen, Qingyuan, Jiangmen and Wenzhou in China. The ITS of nuclear rDNA was amplified from individual <I>A. cantonensis </I>by Polymerase Chain Reaction (PCR) and the representative amplicons were cloned and sequenced. The length of the ITS sequences was 1593-1614 bp for all Chinese <I>A. cantonensis</I> specimens and these sequences were composed of complete ITS-1 sequence of 712-720 bp, complete 5.8 S sequence of 153 bp, complete ITS-2 sequence of 633-650 bp and partial 28 S sequence of 70 bp. The intra-specifc sequence variation in <I>A. cantonensis</I> was 0.1-1.0% for ITS-1 and 0.0-1.3% for ITS-2 whereas sequence comparison revealed that the inter-specifc sequence differences were higher: 15.0-34.6% for ITS-1 and 22.7-24.2% for ITS-2 between <I>A. cantonensis </I>and other <I>Angiostrongylus </I>sp. The results showed that the ITS sequences were conserved among the <I>A. cantonensis</I> isolates however, they were quite different from that of other <I>Angiostrongylus </I>species. Therefore, ITS sequences could provide useful genetic markers for the specific identification and genetic characterization of <I>Angiostrongylus </I>sp.
ER  -