TY  - JOUR
T1  - Molecular Cloning and Sequence Characterization and Tissue Transcription Profile Analyses of Three Novel Common Carp (<I>Cyprinus carpio</I>) Genes-<I>RaB7</I>, <I>RaB8a</I> and <I>RDH1l
AU - Baoliang, BI AU - Lingfu, Kong 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 5
SP  - 585
EP  - 592
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.585.592
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.585.592
KW  - RDH1L
KW  -China
KW  -tissue transcription profile
KW  -Common carp
KW  -RAB7
KW  -RAB8A
AB  - The complete coding sequences of three common carp genes <I>RAB7</I>, <I>RAB8A</I> and <I>RDH1L</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the sequence information of the zebrafish and referenced highly homologous common carp ESTs. The sequence analyses of these three genes revealed that common carp <I>RAB7</I> gene encodes a protein of 204 amino acids which has high homology with the RAB family member rab-7 (RAB7) of fifteen species: Atlantic salmon (97%), zebrafish (91%), sheep (90%), western clawed frog (89%), red jungle fowl (90%), African clawed frog (88%), human (89%), dog (89%), horse (89%), <I>Sumatran orangutan</I> (89%), cattle (89%), rat (89%), mouse (89%), <I>Aiptasia pulchella</I> (88%) and rabbit (87%). The common carp <I>RAB8A</I> gene encodes a protein of 207 amino acids which has high homology with the cmember RAS oncogene family rab8a (RAB8A) of eight species: zebrafish (98%), human (92%), dog (92%), cattle (92%), mouse (91%), rat (91%), <I>Sumatran orangutan</I> (91%) and chicken (87%). The common carp <I>RDH1L</I> gene encodes a protein of 319 amino acids that has high homology with tthe retinol dehydrogenase 1, like (RDH1L) of two species: zebrafish (83%) and Atlantic salmon (65%). Phylogenetic tree analysis revealed that the common carp RAB8A and RDH1L have closer genetic relationships with the zebrafish RAB8A and RDH1L but the common carp RAB7 has a closer genetic relationship with the RAB7 of Atlantic salmon. The tissue transcription profile analyses indicated that that the common carp <I>RAB7</I>, <I>RAB8A</I> and <I>RDH1L</I> genes are generally but differentially expressed in the detected tissues including in tissues including muscle, heart, brain, skin, gills, eye, fin. These data serve as a foundation for further research on these three genes.
ER  -